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DOT drug and alcohol testing

Full DOT-compliant testing for regulated employers

If your team operates under FMCSA, FAA, FTA, or another DOT agency's authority, we handle the full range of required testing — collected and processed to federal chain-of-custody standards under 49 CFR Part 40 and Part 382.

DOT drug and alcohol testing

DOT drug and alcohol testing

Testology, Inc.

DOT authorities we work with

FMCSAFAAFTAFRAPHMSAUSCG

FMCSA — Federal Motor Carrier Safety Administration

FAA — Federal Aviation Administration

FTA — Federal Transit Administration

FRA — Federal Railroad Administration

PHMSA — Pipeline & Hazardous Materials Safety Administration

USCG — United States Coast Guard

Three services, one compliance requirement

DOT drug test

The federally mandated 5-panel urine screen, collected under strict chain-of-custody rules compliant with 49 CFR Part 40.

DOT physical exam

A certified medical examiner's fitness-for-duty exam, entered into the national registry.

DOT breath alcohol test (BAT)

Administered by a trained BAT technician, following the same federally regulated procedure.

Program management

eCCF (Electronic Chain of Custody Form)

A paperless, secure digital form that tracks your specimen from collection to result, reducing errors and speeding up the process.

MRO coordination

Medical Review Officer verification and result delivery for all lab-confirmed tests, as required under 49 CFR Part 40.

FMCSA Clearinghouse reporting

Query and violation reporting handled through the FMCSA Drug & Alcohol Clearinghouse portal on your behalf.

When testing is required

Pre-employment

Required before a safety-sensitive employee performs any covered function for the first time.

Random

Unannounced testing drawn from a pool, at rates set annually by the relevant DOT operating administration.

Reasonable suspicion

Triggered when a trained supervisor observes specific, documented signs of possible substance use.

Post-accident

Required following qualifying accidents involving injury, fatality, or vehicle damage.

Return-to-duty & follow-up

Required after a policy violation, before returning to safety-sensitive work, and periodically after.

Federally mandated panel

DOT testing follows a fixed federal panel under 49 CFR Part 40 — we don't substitute or adjust it, since doing so would invalidate the result for compliance purposes.

DOT 5-PANEL

Frequently asked questions

Common questions from employers managing a DOT compliance program.

Setting up a DOT testing program

We'll walk through your operating administration's specific requirements and get your program compliant — including your random testing pool.

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